电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的洪水泛滥迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：概述放射是否可通过裂解糖酶-β（TGF-β）-依赖性的腺体-间质转换 (EMT)来促进抗体的侵入迁离。使用总量2Gy(60)Coγ线照射是从生命体器官的6种抗体，记录与EMT相关的改变，这包括分别透过显微镜技术，胺基酸印迹依此则，免疫荧光技术，穿孔试验和Transwell小室试验来判读并验证细胞内组织形态，EMT上面，侵入迁离技能等。采行酶酶联免疫吸附依此验证这些抗体之中TGF-β酶技术水平，透过特别抑制剂SB431542来评估TGF-β瞬时通路在放射EMT之中的起着。经过总量为2Gy照射的抗体之中存在间叶细胞内的理解，与实为照射组相比其腺体上面减少，间叶细胞内上面增加，同时其侵入集中于技能提高，TGF-β酶技术水平也提高。进一步发现由A549放射抑止的EMT可通过对TGF-β瞬时抑制发生逆转。这些相比较TGF-β依赖性的EMT在放射抑止提高抗体侵入集中于技能之中起着关键起着。

编辑： lizexiu